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. Author manuscript; available in PMC: 2013 Sep 25.
Published in final edited form as: Biochemistry. 2012 Sep 12;51(38):7515–7524. doi: 10.1021/bi300970h

Figure 1.

Figure 1

Syk peptide biosensor. The biosensor contains a biotinylated substrate reporter that is phosphorylated by Syk, a photocleavable linker for release of the reporter from the biosensor (for future mass spectrometry applications) and a cell penetrating peptide (TAT peptide) for delivery into live cells. Upon delivery of the biosensor to cells and stimulation of the B cell receptor, the substrate reporter is phosphorylated and can be enriched via the biotin tag and analyzed using an antiphosphotyrosine antibody in an ELISA-style multiwell plate format.