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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Mol Microbiol. 2012 Dec 4;87(2):301–317. doi: 10.1111/mmi.12099

Figure 5. Residues T45 and D46 of YopK are important for translocation regulation.

Figure 5

Y. pestis strains carrying the YopM-Bla reporter or the Gst-Bla control, along with either native YopK or YopK variant expression vectors, were used to infect CHO cells at an MOI of 10 for 3 hours. Cells were then incubated with CCF2-AM and analyzed by flow cytometry. Each infection was performed in triplicate, samples were averaged, and standard deviation shown. ANOVA followed by Dunnett post-hoc test was done using the WT infection as the control, and *** indicates P<0.001. White bars: green cells (uninjected), grey bars: aqua cells (low-level injection), black bars: blue cells (high-level injection).