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. Author manuscript; available in PMC: 2014 Jan 8.
Published in final edited form as: Cell Metab. 2012 Dec 27;17(1):113–124. doi: 10.1016/j.cmet.2012.12.001

Figure 5. HIF-1α mediates the effects of AMPK loss on aerobic glycolysis.

Figure 5

A) HIF-1α protein expression in control (Cre−) or AMPKα-deficient (Cre+) MEFs also expressing control or HIF-1α-specific shRNAs. Cells were grown under normoxic conditions (20% O2). AMPKα and actin protein levels are shown. B) Relative expression of pdk1 mRNA in control (Cre−) or AMPKα-deficient (Cre+) MEFs expressing control (Ctrl) or HIF-1α-specific shRNAs. C–F) AMPK-dependent changes in the Warburg Effect are dependent on HIF-1α. C) ECAR of control (Cre−) or AMPKα-deficient (Cre+) MEFs expressing control (Ctrl) or HIF-1α-specific shRNAs grown under normoxic conditions (20% O2). Relative intracellular pyruvate (closed bars) and lactate (open bars) levels (D), glucose consumption (E), and lactate production (F) for cells grown as in (C). **, p<0.01; ***, p<0.001.