Levels of testosterone (T) and its metabolites, dihydrotestosterone (DHT), 5α-androstane-3α,17β-diol (3α-diol), 5α-androstane-3β,17β-diol (3β-diol), 17α-estradiol (17α-E) and 17β-E in the limbic region of male wild type and 3xTg-AD mice at 7 and 24 months. Data are expressed as pg/mg tissue±SEM. The effects of age and genotype and the interaction age-genotype have been analyzed using the two-way ANOVA test. WT-7mo, n=8; WT-24mo, n=5; 3xTg-AD-7mo, n=8; 3xTg-AD-24mo, n=5. * P< 0.05; ** P< 0.01.