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. Author manuscript; available in PMC: 2014 Jan 10.
Published in final edited form as: Mol Cell. 2012 Dec 6;49(1):133–144. doi: 10.1016/j.molcel.2012.11.004

Figure 5. Protein abundance is correlated with population of folding intermediate (I).

Figure 5

(A) Abundance of the insoluble DHFR fraction detected for all lon deleted DHFR mutant strains is correlated to bis-ANS fluorescence measured in vitro at 30°C. (B) Insoluble fraction of the DHFR proteins in lon+ strains does not correlate with bis-ANS fluorescence (see also Figure S6). Insoluble abundance is normalized against V75H+I91L DHFR strain (arbitrary set to 1). (C) Intracellular protein abundance of the soluble DHFR proteins in the strains grown at 30°C inversely correlates with bis-ANS fluorescence, as predicted by Eq. 3. Abundances are normalized against wt DHFR (arbitrary set to 1). bis-ANS fluorescence is normalized against wt DHFR (arbitrary set to 1), see also Table S2. Error bars respresent s.d. of 3 independent experiments.