Fig. 11.
The inflammation-induced shift in spinal GABAA signaling is reversed by intrathecal (i.t.) administration of genistein. Test agents were administered via chronically implanted i.t. catheters to naive and inflamed (CFA) rats. A: mechanical nociceptive threshold was determined before i.t. injection of test agents (testing 1), 20 min after the injection of genistein or genistin (0.1 mg/10 μl, testing 2), and then again 10 min after the injection of muscimol (0.1 μg/10 μl, testing 3). As expected, inflammation was associated with a significant decrease in mechanical threshold. B: a 2-way ANOVA was used to assess the impact of inflammation and tyrosine kinase inhibitor on muscimol-induced changes in nociceptive threshold. The interaction between these 2 factors was significant, with post hoc analysis indicating that this was due to the differential response to muscimol in inflamed rats, which depended on whether or not rats had received an active tyrosine kinase inhibitor. **P < 0.01.