Fig. 7.

GABA_A receptor subunit expression in L₄-L₅ DRG from naive and inflamed rats. A: example of an ethidium bromide-stained gel loaded with the PCR product of each of the GABA_A receptor subunits. A size marker was loaded in the 1st lane, and the subunit amplified is indicated below each subsequent lane; ε_v is a splice variant of the ε-subunit, and “−” is from a control reaction in which the “template” was generated from a reverse transcription reaction in which no reverse transcriptase was added to the reaction mixture. B: pooled data from mRNA harvested from naive and inflamed (CFA) rats amplified with conventional PCR were normalized to expression levels of GAPDH. All subunits were detectable with <35 cycles of amplification, yet there were no detectable differences in relative expression levels between naive and inflamed rats. Real-time PCR (inset) was used to confirm the absence of a detectable change in the expression levels of α₁-, β₂-, and δ-subunits in mRNA extracted from whole DRG from naive and inflamed rats.