Act D (0.5 mg/kg) was administered to wild-type, Abcb1a/1b−/− and Abcc2−/− mice. Blood (A), liver (B), kidney (C) and brain (D) samples were taken at 15 min, 30 min, 2 h and 6 h and Act D concentrations were determined by LC/MS analysis. (A) Inset – semi-logarithmic representation of data. Each point represents mean ± SEM from 3 mice.