Fig. 5.

Role of ERs and MLLs (MLL1–4) on cholesterol uptake in JAR cells. JAR cells were grown up to 60% confluency before transfection with MLL1, MLL2, MLL3, MLL4, ERα, ERβ, and scramble antisense oligonucleotides separately for 48 h. The knocked-down cells were then treated with 100 nm E2 for an additional 4 h and then incubated with 200 μg HDL packaged with tritium-labeled CE for an additional 6 h. Cells were washed with phenol-red-free DMEM four times followed by PBS twice. Cells were then lysed and subjected to liquid scintillation counting for detection of entrapped tritium-labeled cholesterol and plotted (radioactivity uptake). Bars indicate se (n = 4; P ≤ 0.05).