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. 2012 Oct 23;2(1):1–9. doi: 10.1242/bio.20122725

Fig. 2. dMyc physically binds to the miR-308 locus and regulates the transcript levels of miR-308 and its host gene, Ribosomal protein S23 (RpS23).

Fig. 2.

(A) miR-308 locus is shown in the second intron of the RpS23 gene. Arrows in the upstream region of the miR-308 gene indicate the positions of non-canonical E-boxes (CATGTG). PCR products for chromatin-IP are indicated as ChIP region-I and ChIP region-II. (B) Chromatin immuno-precipitation of dMyc by two different anti-dMyc antibodies and IgG, followed by electrophoresis of PCR product. (C) Quantification of ChIP by qPCR. Data are shown as fold enrichment compared to that of the IgG control. Data are presented as means ± standard deviation of three independent biological replicates. (D) Effect of elevated level of Myc on the transcript levels of RpS23, the gene that hosts miR-308. Data are presented as means ± standard deviation of three independent biological replicates.