Fig. 2. Co-localization of GFP-dFMRP mutants with the RNA granules markers deIF4E, dPABP and with poly(A)⁺ mRNA within dFMRP granules in Schneider cells.

Following transfection with the indicated GFP polypeptides, cells were fixed, permeabilized, and then processed for immunofluorescence to detect GFP or GFP-dFMRP (green). The intracellular localization of dPABP and deIF4E (red) is revealed using the appropriate specific antibodies. Scale bar: 10 µm.