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. 2012 Nov 23;31(24):4547–4562. doi: 10.1038/emboj.2012.321

Figure 6.

Figure 6

Mutual independence of Nanog autorepression and OCT4/SOX2-mediated activation. (A) ChIP analysis of OCT4 binding at Nanog in E14Tg2a (n=5). (B) ChIP analysis of SOX2 binding at Nanog in E14Tg2a (n=5). (C, D) ChIP analysis of binding at Nanog after 12 h of Doxycycline treatment of ZHBTc4 cells for OCT4 (C, n=7) and SOX2 (D, n=8). (E) Changes in Nanog mRNA and pre-mRNA levels (n=7) and binding levels of RNAPII (n=3) and TFIIB (n=3) at the Nanog promoter, after 12 h of Doxycycline treatment of ZHBTc4 ES cells (untreated cells set to 1; error bars represent s.e.m.). (F) ChIP analysis of NANOG binding at Nanog after 12 h of Doxycycline treatment of ZHBTc4 cells (n=6). (G–J) ChIP analysis of OCT4 and SOX2 in RCNβH (n=4) and 44NERT (n=3) inducible systems. (K) Relative expression of total Nanog transcripts in ZHBTc4 ES cells (set to one) and in two derivative lines in which the NANOG-ERT2 transgene was randomly integrated (ZNERTc1&2, n=2). (L) Expression of Nanog pre-mRNA in untreated ZNERT clones (Ctl, set to 1) and after 12 h of treatment with the indicated molecules (n=2 for each condition, error bars represent s.e.m.).