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. 2012 Nov 23;32(1):149–158. doi: 10.1038/emboj.2012.303

Figure 4.

Figure 4

SHR-driven PIN3 stele expression is not sufficient to rescue pin3 LRI phenotype. (A) The SHR promoter drives PIN3-YFP expression (green) in the stele of the RM, EZ and DZ and during LRI (arrowheads) in the pin3/DR5::GFP background, with PI counterstain (red) in the upper panel. Colour-coded asterisks indicate the different root cell files. Note that PIN3-YFP is also present in the dividing pericycle cells during LRI. The fluorescent signal in the root cap is derived from the DR5::GFP marker. Scale bar: 50 μm. (B) Stage distribution of LR primordia indicates that the decrease in stage I is not restored in pin3 mutants when PIN3-YFP is expressed in the stele. +SHR and –SHR refer to the pooled fluorescent-positive and -negative seedlings, respectively, from a segregating population in the stable pin3/DR5::GFP background. Error bars represent s.e.m. (n=10–20). (C) The SHR promoter drives PIN3-YFP expression in the dividing pericycle cells from the first stage of LR development onwards. Corresponding stages of LR development are indicated. The PI counterstain is shown in red. Scale bar: 50 μm.