Figure 5.

Search for alternative FLC gene loops induced by vernalization. (A) Quantitative 3C analysis using FIV as the anchor region. (B) Quantitative 3C analysis using FV as the anchor region. Col-FRI seedlings were harvested after 10 days of growth in standard conditions (NV), immediately after 2 weeks in the cold (2WT0) and after 2 weeks in the cold and 7 days of further growth in the warm (2WT7). Relative interaction frequencies were calculated as described in Materials and methods. The data are the average of two biological replicates. In the graphs, BamHI and BglII restriction sites are indicated with vertical dotted lines, the analysed FLC regions are numbered with Roman numerals and the anchor region is highlighted with a grey-shadowed area. A schematic representation of the FLC locus is shown above each graph.