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. 2012 Jul 5;22(2):268–278. doi: 10.1089/scd.2012.0222

FIG. 2.

FIG. 2.

Effects of p53 knockdown and hTERT overexpression on proliferation, colony formation, and differentiation of MSCs. (A) Effects of p53 knockdown and hTERT overexpression on proliferation of MSCs. Bars show the mean±SD cells from 2 patients, tested twice. Asterisks indicate a significant difference compared with control MSCs, showing that p53 knockdown alone or combination of p53 knockdown and hTERT overexpression promoted MSC proliferation, whereas there was no significant difference in proliferation between hTERT-MSCs and control MSCs at passage 5 (*P<0.05). (B) Effects of p53 knockdown and hTERT overexpression on colony formation of MSCs. About 500 MSCs at passage 5 were seeded in 10-cm dishes; the colonies were counted after Giemsa stain 14 days after seeding. Asterisks indicate a significant difference compared with control MSCs, showing that p53 knockdown alone or combination of p53 knockdown and hTERT overexpression enhanced colony formation of MSCs (*P<0.05). (C) Effects of p53 knockdown and hTERT overexpression on adipogenic markers and osteogenic markers during differentiation of MSCs at passage 5 by quantitative real-time polymerase chain reaction (PCR). Bars show the mean±SD fold change in transcript expression in MSCs from 2 patients, tested twice, relative to control MSC (arbitrarily set at 1, *P<0.05). (D) p53 knockdown and hTERT overexpression enhanced oil red stain for lipoid deposits in adipogenesis and AP stain for alkaline phosphatase in osteogenesis of MSCs at passage 5.