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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: J Cell Biochem. 2009 May 1;107(1):76–85. doi: 10.1002/jcb.22102

Figure 2.

Figure 2

Induction of apoptosis in human lung H5810 or prostate PC3 cancer cells. a. After infected H5810 cells with V12ras, immunoblotting was performed to detect Ras expression. Equal loading of total proteins was verified by β-actin expression. b. ras loop mutant genes were infected into H5810 cells and the expressions were tested by immunoblotting. Equal loading of total proteins was verified by β-actin expression. c. H5810 cells expressing V12ras, and the loop mutant genes were treated with wortmannin (1.0 μM) or KP372-1 (1.0 μM) for 15 min, prior adding GO6976 (1.0 μM for 48h). Subsequently, DNA fragmentation assay was conducted. Error bars represent the standard deviation over five independent experiments. d. PC3 or prostate epithelial PrEC cells were treated with wortmannin (1.0 μM) or KP372-1 (1.0 μM) for 15 min, prior adding GO6976 (1.0 μM, 48h). Subsequently, DNA fragmentation assay was conducted. Error bars represent the standard deviation over five independent experiments.