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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: J Cell Biochem. 2009 May 1;107(1):76–85. doi: 10.1002/jcb.22102

Figure 4.

Figure 4

Activation of ER stress regulators following downregulation of PKC. a. After treated with GO6976, the expression of p-PERK in the cells ectopically expressing V12ras or the loop mutant genes was examined by immunoblotting. Equal loading of total proteins was verified by PERK expression. b. Cells were treated with various inhibitors for 15 min prior to adding GO6976 and then immunoblotted for p-PERK. Equal loading of total proteins was verified by PERK expression. c. Following PKC suppression, RLE cells expressing v-ras or the loop mutant genes were immunoprecipitated with anti- PERK, ATF6 or IRE1 antibodies. After took out the immuno-complexes, equal amount of the supernatants were immunoblotted for the expression of BiP.