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Schematic of microbead incubation. (1) Prior to assay, the magnetic microbeads are conjugated with an antigen-specific antibody. (2) After the first incubation stage, the antigen is bound to each bead in an amount proportional to the sample concentration. (3) In the second incubation stage, the microbeads are incubated with a fluorescently tagged secondary antibody to enable fluorescence detection of the antigen concentration.
