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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: J Immunol. 2012 Nov 19;189(12):5498–5502. doi: 10.4049/jimmunol.1102150

Figure 2.

Figure 2

Il13ra1 enhances neuronal loss in vivo and in vitro. A, Il13ra −/Y mice show decreased DA neuron loss in a chronic peripheral inflammatory model of PD. Histogram of the total number of TH positive neurons in the SNc of Il13ra −/Y and +/Y mice chronically injected (i.p.) with LPS (100μg/kg) twice weekly for six months versus control mice injected with saline used as vehicle (n=11 mice/group). Values (mean +/− SEM) were normalized to the Il13ra +/Y/saline group and asterisk indicates p < 0.05 versus all other groups. B, Representative images showing immunohistochemical labeling of DA neurons using a TH antibody for Il13ra −/Y and +/Y mice injected with saline or LPS as indicated. Scale bar = 200 μm. C, IL-13 and IL-4 potentiate oxidative stress induced cell death in MN9D cells. Cells were treated with saline, IL-13 or IL-4 at the doses indicated followed by H2O2. Cell viability was assessed by the LDH assay 24 h later. Values (mean +/− SEM) are normalized to PBS only group and are from three independent experiments. Asterisks indicate p < 0.05 versus saline or 80 μM H2O2 group. D, Similar experiments were carried out in the presence of tBOOH.