Figure 7.

Muscarinic-mediated recruitment of UNC-13/Munc13 requires SPHK-1. a, Representative images (left) and quantification (right) of GFP-tagged UNC-13S (UNC-13S-GFP) in axons of DA/DB motor neurons analyzed in the indicated strains. *p < 0.05 and ***p < 0.0005, Student's t tests. Scale bar: 10 μm. b, Model of the muscarinic- and calcium-mediated SPHK-1 translocation for the facilitating effects of muscarinic signaling pathways on neurotransmitter release in C. elegans cholinergic motor neurons. Dotted lines represent unknown pathways or pathways that are likely to be connected via multiple proteins.