Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1975 Apr;15(4):1042–1046. doi: 10.1128/jvi.15.4.1042-1046.1975

Purification and properties of a bacteriophage T5-modified form of Escherichia coli RNA polymerase.

C Szabo, R W Moyer
PMCID: PMC354552  PMID: 1090747

Abstract

A modified form of Escherichia coli RNA polymerase that contains one of the three T5-specific polypeptides known to interact with the host enzyme was purified from bacteriophage T5-infected cells. The properties of this T5-modified enzyme appeared identical to those of the RNA polymerase derived from uninfected non-colicinogenic cells and to a fully active enzyme isolated from T5-infected ColIb+ cells after the limited in vivo transcription of T5 genes allowed by the plasmid had ceased.

Full text

PDF

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burgess R. R. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6160–6167. [PubMed] [Google Scholar]
  2. Cooper T. G. Procedure for uniform binding of DNA to nitrocellulose filters. Anal Biochem. 1972 Jun;47(2):481–486. doi: 10.1016/0003-2697(72)90141-8. [DOI] [PubMed] [Google Scholar]
  3. Lembach K. J., Buchanan J. M. The relationship of protein synthesis to early transcriptive events in bacteriophage T4-infected Escherichia coli B. J Biol Chem. 1970 Apr 10;245(7):1575–1587. [PubMed] [Google Scholar]
  4. Moyer R. W., Buchanan J. M. Effect of calcium ions on synthesis of T5-specific proteins. J Biol Chem. 1970 Nov 25;245(22):5897–5903. [PubMed] [Google Scholar]
  5. Moyer R. W., Buchanan J. M. Patterns of RNA synthesis in T5-infected cells. I. As studied by the technique of DNA-RNA hybridization-competition. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1249–1256. doi: 10.1073/pnas.64.4.1249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pispa J. P., Buchanan J. M. Synthesis of bacteriophage T5 specific RNA in vitro. Biochim Biophys Acta. 1971 Sep 30;247(1):181–184. doi: 10.1016/0005-2787(71)90823-9. [DOI] [PubMed] [Google Scholar]
  7. Schachner M., Seifert W., Zillig W. A correlation of changes in host and T 4 bacteriophage specific RNA synthesis with changes of DNA-dependent RNA polymerase in Escherichia coli infected with bacteriophage T 4 . Eur J Biochem. 1971 Oct 26;22(4):520–528. doi: 10.1111/j.1432-1033.1971.tb01572.x. [DOI] [PubMed] [Google Scholar]
  8. Stevens A. Deoxyribonucleic acid dependent ribonucleic acid polymerases from two T4 phage-infected systems. Biochemistry. 1974 Jan 29;13(3):493–503. doi: 10.1021/bi00700a015. [DOI] [PubMed] [Google Scholar]
  9. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES