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. 2013 Feb;27(2):684–691. doi: 10.1096/fj.12-218073

Figure 3.

Figure 3.

PTU impairs normal Pitx2c and Coco expression. A) PTU resulted in predominately absent Pitx2c expression in the left LPM. Note that parallel left-sided knockdown of Coco rescued left LPM expression of Pitx2c. PTU prevented left-sided down-regulation of Coco in postflow neurula stage embryos. Numbers in parentheses represent number of analyzed specimens, derived in each case from ≥3 independent experiments. B) ISH (st. 19) of dorsal explants for the early LR marker Coco. Embryos were treated with vehicle or PTU from st.8 to 19. Vehicle treatment resulted in normal expression of Coco (R>L). PTU treatment caused different expression of Coco. R > L, normal Coco expression; R = L, equal Coco expression; R < L, reversed Coco expression. ***P < 0.001; 1-way ANOVA.