Table 1.
Antibodies used in this report
| Antibody | Vendor | Catalog no. | Application/working dilution |
|---|---|---|---|
| Rabbit anti-N-cadherin | Santa Cruz Biotechnology (Santa Cruz, CA, USA) | sc-7939 | IB (1:200) |
| Rabbit anti-α-catenin | Santa Cruz Biotechnology | sc-7894 | IB (1:200) |
| Rabbit anti-β-catenin | Santa Cruz Biotechnology | sc-7199 | IB (1:200) |
| Rabbit anti-nectin-3 | Santa Cruz Biotechnology | sc-28637 | IB (1:200) |
| IP (2 μg IgG/reaction) | |||
| Goat anti-nectin-3 | Santa Cruz Biotechnology | sc-14806 | IF (1:50) |
| Rabbit anti-afadin | Sigma (St. Louis, MO, USA) | A 0349 | IB (1:500) |
| Goat anti-JAM-C | R&D Systems (Minneapolis, MN, USA) | AF1213 | IB (1:500) |
| Rabbit anti- p-FAK-Tyr397 | Thermo Scientific (Waltham, MA, USA) | PA1-22001 | IB (1:1000) |
| IF (1:50) | |||
| Rabbit anti-FAK | Santa Cruz Biotechnology | sc-558 | IB (1:500) |
| Mouse anti-GAPDH | Abcam (Cambridge, MA, USA) | ab8245 | IB (1:1000) |
| Mouse anti-Arp3 | Sigma | A5979 | IB (1:3000) |
| IF (1:150) | |||
| Rabbit anti-p-Tyr | Life Technologies (Carlsbad, CA, USA) | 61-5800 | IB (1:500) |
| Rabbit anti-sFRP1a | Cell Signaling Technology (Danvers, MA, USA) | 4690 | IB (1:1000) |
| Rabbit anti-sFRP1 | Santa Cruz Biotechnology | sc-7425 | IB (1:200) |
| Rabbit anti-testin | Cheng et al. (73) | / | IB (1:1000) |
IB, immunoblotting; IP, immunoprecipitation; IF, immunofluorescence microscopy. aAnti-sFRP1 antibodies from Cell Signaling Technology and Santa Cruz Biotechnology detected overexpressed sFRP1 in both lysates and conditioned medium from Sertoli-germ cell cocultures; however, results shown in Fig. 6A were obtained using anti-sFRP1 antibody from Cell Signaling Technology.