Fig. 4. Relative IL-12 family cytokine gene expression in WAT during the progressive development of genetic and diet-induced obesity.
Relative mRNA abundance of IL-12 family cytokine (A) and inflammatory genes (B) was determined by qRT-PCR from total RNA extracted from white adipose tissue (WAT) at progressive stages of obesity from ob/ob mice as well as mice subject to diet-induced obesity (DIO). Data were normalized to 18S and relative abundance determined for each cytokine where obese values were expressed as fold-differences relative to lean within each group. Differences in gene expression between lean and obese animals were determined via student’s t-test where a p-value of <0.05 was considered significant.