Table 1.
Name | Accession | ABI number | a WAT CT | b RAW CT | c PA CT | d AD CT | ||||
---|---|---|---|---|---|---|---|---|---|---|
IL-12 cytokines | IL-27 | IL-35 | IL-12 | IL-23 | ||||||
p28 | • | NM_145636 | Mm00461164_m1 | 32 | 28 | 34 | 28 | |||
EBI3 | • | • | NM_015766 | Mm00469294_m1 | 28 | 24 | 32 | 34 | ||
p35 | • | • | NM_008351 | Mm01208555_m1 | 32 | 35 | 36 | 35 | ||
p40 | • | • | NM_008352 | Mm00434174_m1 | 30 | 33 | 36 | 36 | ||
p19 | • | NM_031252 | Mm00518984_m1 | 27 | 25 | 30 | 31 | |||
IL-12 receptors | ||||||||||
WSX-1 | • | NM_016671 | Mm00497259_m1 | 28 | 34 | 33 | 36 | |||
gp130 | • | NM_010560 | Mm00439665_m1 | 21 | 25 | 22 | 24 | |||
IL-12Rβ2 | • | NM_008354 | Mm00434200_m1 | 29 | 33 | 35 | 36 | |||
IL-12Rβ1 | • | • | NM_008353 | Mm00434189_m1 | 29 | 29 | 30 | 34 | ||
IL-23R | • | NM_144548 | Mm00519943_m1 | 28 | 32 | 33 | 36 | |||
adipocyte and inflammatory genes | ||||||||||
Adipsin | NM_013456 | Mm00442664_m1 | 17 | ND | 35 | 14 | ||||
MCP-1 | NM_011333 | Mm00441242_m1 | 27 | 17 | 23 | 27 | ||||
IL-6 | NM_031168 | Mm99999064_m1 | 32 | 26 | 29 | 30 | ||||
TNFα | NM_013693 | Mm99999068_m1 | 29 | 21 | 34 | 35 | ||||
reference gene | ||||||||||
18S | X03205 | 4342930E | 9 | 7 | 7 | 8 |
Average threshold cycle (CT) for white adipose tissue (WAT) from 10 wk old, lean C57BL/6J mice.
Average threshold cycle (CT) for confluent murine RAW 264.7 macrophage cells.
Average threshold cycle (CT) for confluent murine 3T3-L1 preadipocytes (PA).
Average threshold cycle (CT) for mature day 8 murine 3T3-L1 adipocytes (AD).
ND = non-detectable threshold cycle.