Fig. 5.

Toxicity of resveratrol derivatives to mammalian cells. HEK293 cells were seeded on six-well plates and allowed to attach overnight. The following day, resveratrol, Derivative 1 (3,5-hydroxy-4′-thiomethyl-stilbene), Derivative 2 (3,5-hydroxy-4′-methyl-stilbene), or Derivative 3 (3,5-hydroxy-4′-ethyl-stilbene) was added at a concentration of 50 μm. Cells in replicate wells were periodically washed to remove any debris or detached cells, trypsinized, and counted using a Beckman-Coulter Z2 particle counter. The proportion surviving is plotted as a percentage of cells counted in the corresponding ethanol (vehicle)-treated wells.