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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: J Immunol. 2010 Jul 16;185(4):2080–2088. doi: 10.4049/jimmunol.1000532

FIGURE 3.

FIGURE 3

mDCs are required for NK cells activation in response to dsRNA. NK cells activation was evaluated by CD107a staining (upper panels) and the IFN-γ production (lower panels), as described (Fig. 1). A, A total of 2 × 106/ml whole human PBMCs or depleted in myeloid cells (CD33), monocytes (CD14), pDCs (BDCA4), or mDCs (both BDCA1 and BDCA3 subtypes) were activated with the indicated concentrations of p(IC) and p(AU). B, 1 × 106/ml MACS-purified human NK cells were activated with the indicated concentrations of p(IC) and p(AU) with or without 2 × 105/ml MACS-purified blood mDC (both BDCA1 and BDCA3 subtypes). Data from A and B represent independent values of four and three different donor, respectively. C, RIG-I, MDA5, and TLR3 mRNA levels were determined in the three different cell types using real-time PCR. Results are expressed as arbitrary units normalized to value for the housekeeping gene GAPDH. Data represent independent values of four to six different donors and horizontal bars the median. *p < 0.05; ***p < 0.001. p(AU), poly(A:U); p(IC), poly(IC).