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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Exp Eye Res. 2012 Oct 29;106:47–54. doi: 10.1016/j.exer.2012.10.006

FIGURE 6.

FIGURE 6

Confocal immunofluorescence analysis of mitochondrial localization of P-gp (A-C) and PepT-1 (D-F) proteins. rPCECs cultured on glass coverslips were incubated with 5 nm of MitoTracker® red to label mitochondria (red), fixed and processed for P-gp protein (green) using mdr-1 mouse monoclonal IgG or PepT-1 protein (green) using pepT-1 rabbit polyclonal IgG. (A) P-gp (green), (B) Mitochondria (red), (C) Image of colocalized points (yellow) between red (mitochondria) and green (P-gp), (D) PepT-1 (green), (E) Mitochondria (red), (F) Image of colocalized points (yellow) between red (mitochondria) and green (PepT-1). Fluorescence signals obtained using ImageJ colocalization Plugin Software (NIH). The images are representative of at least three independent experiments with similar results. Mean value of Pearson’s correlation coefficient (Rr) = 0.58 (A-C), 0.69 (D-F) and Overlap coefficient (R) = 0.99 (C, F).