Figure 3.

Immunostaining for Ctrc in mouse molars. Twelve-day-old mouse mandibles were fixed in 10% formalin, demineralized for 7 days in 10% EDTA, embedded in paraffin, and sectioned. Following imunohistochemistry protocols as described (Lacruz et al., 2013), sagittal sections of molars were immunostained with Ctrc antibody (ab35694, Abcam, Cambridge, UK). Ctrc is expressed at the apical pole of maturation-stage ameloblasts (Am) but not in the enamel matrix (E). Secretory ameloblasts did not stain for Ctrc (data not shown).