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. 2001 Jul 3;98(14):8000–8005. doi: 10.1073/pnas.141229598

Figure 2.

Figure 2

Expression of C/EBPα but not C/EBPɛ in 32Dcl3 cells overexpressing CCAAT displacement protein (CDP) induced with G-CSF. (A) 10 μg of RNA isolated from uninduced (lanes 1 and 3) and G-CSF-induced (lanes 2, 4, and 5) CDP/cut overexpressing 32Dcl3 (CDP+) and Vector alone 32Dcl3 (CDP−) cells were subjected to Northern blot analysis. The Northern blot was probed with a 32P-labeled cDNA probe for C/EBPɛ (Top). (B) Northern blot analysis of the same RNAs probed by using a 32P-labeled C/EBPα probe revealed G-CSF-induced expression of this transcription factor in both CDP overexpressing 32Dcl3 (CDP+) (Top, lanes 3, 4 and 5) and Vector alone 32Dcl3 (CDP−) cells (Top, lanes 1 and 2). The level of lactoferrin (LF) mRNA was up-regulated only in the G-CSF-induced CDP− cells (Middle, lanes 1 and 2), but not in similarly treated CDP+ cells (Middle, lanes 3, 4, and 5). Equal loading of RNA in each lane of each of the two blots was determined by probing the blots with β-Actin (Bottom).