Figure 4.

EMSA analysis of the CDP/cut site in the C/EBPɛ promoter. Electrophoretic mobility shift analysis was carried out by using ³²P-labeled double-stranded oligos encoding the CDP/cut site in the C/EBPɛ promoter. Addition of nuclear extracts prepared from K562 cells resulted in the formation of a protein–DNA complex (lane 2, see arrow), which was specifically competed away by the addition of a 100-fold molar excess of unlabeled self (lane 3, Self comp.), or known CDP binding oligos—i.e., NCAM (lane 4) and E36 (lane 5). Preincubation of the nuclear extracts with anti-CDP serum (lane 7), but not with preimmune serum (lane 6), resulted in the loss of the protein–DNA complex.