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Sensitivity of Ln-PCR against serial dilution of cultured promastigote DNA seen in 1.5%agarose gel electrophoresis. Lanes 1 to 7 contain cultured promastigote L. donovani DNA of 100 pg, 10 pg, 1 pg, 100 fg, 10 fg, 1 fg and 0.1 fg respectively; Lane 08 = 100 bp ladder and Lane 09 = negative control (PCR grade water)
