Table 1.

Dissociation constants of various sugars and their EC₅₀ for vir gene expression in A. tumefaciens wild-type strain A348 and mmsB deletion strain AB520

Sugars	Kd*, mM	EC50†, mM
		A348	AB520
Arabinose	5.92E-04	3.3	3.0E-04
Galactose	3.34E-04	2	4E-03
Galacturonic acid	0.13	0.08	0.03
Glucuronic acid	0.031	0.4	0.4

*K_d from ITC as described in Fig. S1 and Table S2.

^†The EC₅₀ is the concentration of the sugar that yielded one-half of the maximal activity in an assay of β-galacotsidase activity from lacZ driven by the virB promoter on plasmid pSW209Ω.