Fig. 5.

Effect of agonists on receptor mobility. (A–C) Cells were transfected with SNAP-β₁AR (A), SNAP-β₂AR (B), or SNAP-GABA_B1 plus wild-type GABA_B2 (C) constructs, labeled with Alexa647-BG, and stimulated with the indicated concentrations of agonists 5–10 min before image acquisition. Shown are the distributions of diffusion coefficients of the analyzed receptor particles compared with control nonstimulated cells. Differences in C are statistically significant by a Mann–Whitney test (P < 0.0001).