Fig. 5.

scl mRNA injection rescues hematopoietic deficiency caused by reduction in Hh signaling (cyc) and/or reduction in notch signaling (DAPT) from the 10-somite stage. Embryos were fixed at 36 hpf and analyzed by whole-mount in situ hybridization for (i) c-myb, (ii) runx1, (iii) flk1, (iv) vegfa, and (v) ephrinb2a. For runx1 and flk1 expressions, lateral views of the trunk are accompanied by dorsal views on the right. The ratios indicate the number of embryos out of the total that show similar staining to the representative picture shown. The ratio within the scl injection represents the number of embryos that show an increase in staining over the vehicle control. The red arrows highlight ephrinb2a expression.