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. 2012 Dec 24;110(2):543–548. doi: 10.1073/pnas.1214883110

Fig. 5.

Fig. 5.

Synchrony of spermatogenesis in Rdh10 sgKO testes after RA treatment. (A and B) Hematoxylin staining of representative testes from 3-wk-old control (A) and Rdh10 sgKO (B) mice with a vitamin A-containing diet for 5 wk, after an RA injection. Roman numerals indicate the stage of the seminiferous epithelial cycle. (C and D) Immunostaining for STRA8 (red) in sections of representative testes from 3-wk-old control (C) and Rdh10 sgKO (D) mice after RA treatment. The seminiferous epithelium contains STRA8-positive cells, a characteristic marker of stage VIII. (E) Stage frequency of seminiferous epithelial cycle in control and Rdh10 sgKO individuals after RA treatment. (F) Calculated synchronization factors of control and Rdh10 sgKO mice after RA treatment. n = 5–8. Error bars represent SD. *P < 0.05, Student t test. (Scale bars: 100 μm.)