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. 2012 Dec 26;110(2):779–784. doi: 10.1073/pnas.1214287110

Fig. 1.

Fig. 1.

Screening of T-DNA insertion mutant lines for the detection of changes in CMV susceptibility. (A) The mutant #75010 infected with CMV showed a much healthier growth phenotype than the CMV-infected Col-0 plant at 18 dpi. (B) The apum5-D mutant showed an up-regulated gene expression compared with Col-0 in 3-wk-old plants, as shown by qRT-PCR analysis. Significant difference (Student t test; *P < 0.05) is indicated with an asterisk. (C) ELISA for CMV coat protein accumulation detection in Col-0 and apum5-D mutant. Significant difference (Student t test; *P < 0.05) is indicated with an asterisk. (D) RNA blot analysis for CMV detection in Col-0 and apum5-D mutant plants. CMV-inoculated plant RNA was extracted and RNA blot analysis was performed using 3′ UTR probes specific to CMV. rRNA was used as a loading control. The relative band intensity was quantified by using the ImageJ software. (E and F) Analysis of fresh weight and stem length in CMV-inoculated Col-0 and apum5-D mutant plants at 18 dpi. Significant differences (Student t test; *P < 0.05, **P < 0.01) are indicated with asterisks. Error bars show mean ± SD (n = 12).