Fig. 4.

Role pf the NEDD8 pathway in the development of allergic inflammation. (A) GFP⁺CD4⁺ T cells from OT-II control or OT-II Ubc12 knockdown chimeric mice were adoptively transferred into C57BL/6J recipient mice, followed by immunization with OVA plus alum (n = 3). Starting at 10 d after immunization, the mice were challenged intranasally with OVA protein for the next 4 d. The mice were examined at 24 h after the last challenge. (B) Lungs from control mice (Left) and shUbc12 knockdown chimeric mice (Right) were sectioned and stained with H&E to visualize inflammatory infiltrates. (C) Total numbers of eosinophils (Eos), neutrophils (Neu), monocytes (Mon), and lymphocytes (Lym) were calculated by FACS analysis. (D) IL-4 (Left) and IL-5 (Right) concentrations in BAL fluid were measured by ELISA at 24 h after the final OVA challenge. The results are representative of two repeated experiments.