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. 2013 Jan 15;11(1):e1001467. doi: 10.1371/journal.pbio.1001467

Figure 3. Wnt1 and Wnt8 signaling are necessary for Fzl5/8-JNK-mediated ANE restriction.

Figure 3

(A) Three-color in situ hybridization for wnt1 (red), wnt8 (green), and foxq2 (magenta) transcripts during ANE restriction. wnt1 mRNA appears yellow when overlapping with wnt8 mRNA. (B) The foxq2 expression domain is not restricted in embryos injected with a Wnt1 (b) or Wnt8 (c) morpholino. (C) foxq2 expression is completely eliminated in embryos injected with either Wnt1 (b) or Wnt8 (d) mRNA. The Wnt1- and Wnt8-mediated inhibition of foxq2 expression requires functional Fzl5/8 (c, e); the Wnt8-mediated inhibition of foxq2 expression requires JNK activity (f). MO, morpholino.