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. 2013 Jan 15;8(1):e53831. doi: 10.1371/journal.pone.0053831

Figure 5. Effect of atrogin-1 on inflammation.

Figure 5

A. Neonatal rat cardiomyocytes were infected with Ad-GFP or Ad-atrogin-1-GFP for 24 h and then treated with LPS (1 µg/ml) for additional 24 hours. The qRT-PCR analysis of gene expression was performed in triplicate using specific oligonucleotides primers. Data represent the mean ± SEM. # P<0.05, & P<0.01, $ P<0.05 vs. Ad-GFP; *P<0.01 vs. Ad-GFP+LPS. B. Neonatal rat cardiomyocytes were infected with Ad-siRNA-control or Ad-siRNA-atrogin-1 for 24 h and then treated with LPS (1 µg/ml) for additional 24 hours. The qRT-PCR analysis of gene expression was performed as in A. Data represent the mean ± SEM. # P<0.05, & P<0.01, $ P<0.05 vs. siRNA-control; *P<0.01 vs. Ad-siRNA+LPS.