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. 2013 Jan 15;8(1):e53988. doi: 10.1371/journal.pone.0053988

Figure 3. MGP inhibits transformed activation of HSCs.

Figure 3

Isolated HSCs were incubated in the presence or absence of 200 µg of MGP for 1, 3, and 7 days. After treatment, (A) the cell morphology was investigated by phase-contrast microscopy, and (B) the expression levels of α-SMA and collagen I after 7 days incubation were examined by immunostaining. (C) MGP suppressed α-SMA and collagen I expression in activated HSCs. The activated HSCs (5 passages) were incubated without or with 200 µg of MGP for 1 and 3 days. After incubation, the expression levels of α-SMA and collagen I were examined by immunoblotting. (D) The expression of α-SMA and collagen I after 3 days incubation was investigated by immunostaining.