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. 2013 Jan 15;8(1):e54290. doi: 10.1371/journal.pone.0054290

Figure 4. Furin processing of integrin αV.

Figure 4

The original glioma U251 cells, breast carcinoma MCF-7 cells with the enforced expression of the β3 integrin subunit (MCF7-β3), colon carcinoma LoVo cells with and without the enforced expression of furin and CHO cells with the enforced expression of the integrin αV precursor (pro-αV) or the furin-resistant pro-αV mutant (pro-αV-A889A890) were cell-surface biotinylated and then lysed. The lysates were precipitated using the αVβ3 antibody LM609 and analyzed by Western blotting with Extravidin-horseradish peroxidase. Where indicated, cells were co-incubated for 16–18 h prior to biotinylation with a synthetic furin inhibitor (DEC; 50 μM).