Figure 5. Adherence of WT 5448 and 5448Δshr to HEp-2 and HaCaT cells. Confluent HEp-2 (A) and HaCaT (B) monolayers were incubated with mid-log phase bacterial cultures at 37°C and 5% CO₂ for 30 min. After washing unattached bacteria and lysing the cells, the percent binding was determined by the dividing the number of bound bacteria per well by the starting inoculum and multiplying by 100%. Each bar shows the mean ± SEM.