Table 1.
Checkerboard test results
Elk | Caribou | Bison | |
---|---|---|---|
Protein G dilution (a) |
1:16000 |
1:8000 |
1:16000 |
Anti-deer dilution |
1:250 (b) |
1:175 (c) |
NT |
Sample dilution using Protein G |
1:30 |
1:20 |
1:160 |
Sample dilution using anti-deer | 1:30 | 1:5 | NT |
(a) Concentration not available from manufacturer.
(b) 0.40 μg/ml.
(c) 0.57 μg/ml.
Optimal dilutions of Protein G and anti-deer conjugates when used with serum from elk, caribou or bison, and optimal species-specific serum dilutions for use with the IDEXX Mycobacterium paratuberculosis Antibody Test. NT: not tested.