Figure 5. Adjustment of Tsc1^cko mutant ovaries by postnatal rapamycin treatment.

(A) Decreased activity of mTORC1 in Tsc1^cko mutant ovaries after rapamycin treatment as indicated by down-regulated phosphorylated rpS6; rpS6 and tubulin were used as control. (B) The Tsc1^cko mutant ovaries treated with rapamycin were lighter than those treated with vehicle. (C and D) Hemaxytoxylin and eosin staining showed that the folliculogenesis of Tsc1^cko mutant ovaries declined after rapamycin treatment compared with controls. (E) The rapamycin treated Tsc1^cko mutant mice ovulated less oocytes than Tsc1^cko mutant mice treated with vehicle after superovulation. Bar, 250 µm. *,P<0.05.