Figure 3.

Ceramide-dependent signalling steps are associated with lung inflammation in mice. Activation of signalling steps in lung homogenate and induction of inflammation determined in BAL in mice (n = 5) treated with PBS, CNP [5 mg/kg bodyweight], or CNP together with ectoine [E, 1 mM]. (A) Phospho-EGFR at Tyr¹¹⁷³ signals relative to total EGFR signals. (B) Phospho-SFK at Tyr⁴¹⁶ signals relative to GAPDH signals. (C) Neutrophilic granulocytes in BAL (D) KC in BAL. *, Significantly different from control; †, significantly different from CNP alone (p < 0.05).