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. 2013 Jan 15;8(1):e53347. doi: 10.1371/journal.pone.0053347

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© 2013 Müller et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Dimerization studies were performed using sandwich ELISA. COS-7 cells were transiently transfected. As negative control serves the NHA-hGHSR (white column) and as positive control the co-transfection of NHA-tagged hMC3R and FLAG-tagged hGHSR (black column, [28]). The light grey column represents the average of the HA- respectively FLAG-tagged mGPR83 in combination with the correspondent tagged rM3R. The dark grey column represents co-transfection of HA-tagged mGPR83 and FLAG-tagged mGPR83. Dimerization was measured via the HA epitope. The mean absorption (492 nm/620 nm) is calculated per 1 mg/ml of protein and shown as percentage of the hMC3R/hGHSR heterodimer (absorption (492/620)/ 1mg/ml of protein: 0.3 ± 0.04). Data were assessed from 3 independent experiments, each performed in triplicates and represent mean ± SEM.