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Effect of 20E on dacapo abundance. Cells were seeded as detailed in the Materials and Methods and incubated in the presence (+) or absence (−) of 2×10−6M 20E for 24 or 48 h. For cells in lanes 1, 2, 5, and 6 20E was added to the growth medium; in lanes 3, 4, 7, and 8 the medium was replaced with fresh E-5 medium containing 2×10−6 M 20E.
