Figure 3. Specific detection of DSG3 in human cervical lymph nodes.

A. Formalin fixed and paraffin embedded tissue sections of non-metastatic (N−) and metastatic lymph nodes (N+) show DGS3 expression only in N+, with the staining localized to the malignant squamous cells (n=30). All N− cases were negative (n=5). B. The epithelial specificity of DSG3 immunoreactivity was further confirmed using simultaneous cytokeratin (CK) staining. A representative example is shown, whereby the H&E stained tumor island is matched with CK and DSG3 expression, with no non-specific staining. An example of a N− case stained for DSG3 is shown.