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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Oral Oncol. 2012 Sep 23;49(2):93–101. doi: 10.1016/j.oraloncology.2012.08.001

Figure 5. Detection of DSG3 in metastatic human cervical lymph nodes.

Figure 5

H&E stained cryosections of representative non-metastatic (−) and metastatic (+) human cervical lymph nodes were scanned and the total number of tumor cells per section was quantified (Table 1). Serial sections of these lymph nodes were evaluated by immunofluorescence for DSG3 and detected only in metastatic lymph nodes (green). Vimentin (red) was used to identify stromal tissue, and nuclei of all cells were stained blue with DAPI (Fig. 5A). Protein extracts made from single cryosections of lymph nodes were used for the detection of DSG3 by Western blot analysis and DSG3 quantification using nanosensors. DSG3 levels were similar to background for all non-metastatic samples, while DSG3 levels in all metastatic cases were proportional to the number of invading HNSCC cells (Fig. 5B).